L3397

LB Broth (Luria low salt)

Powder microbial growth medium

• Synonyme(s) : Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
• Code UNSPSC : 41106200
• Nomenclature NACRES : NA.85

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Stérilité: non-sterile
• Forme: powder
• Composition: NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• Technique(s): microbiological culture: suitable
• pH: 6.8-7.2(1.55% solution)
• Adéquation: suitable for molecular biology; nonselective for Escherichia coli; nonselective for coliforms
• Application(s): food and beverages; microbiology
• Température de stockage: room temp

Description

Description générale

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Application

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Caractéristiques et avantages

Luria′s low salt LB powder provides:

• Standard formulation
• A budget-friendly alternative to liquid
• Easy scale-up using larger package sizes

Notes préparatoires

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Reconstitution

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable