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Merck

L1900

Luria broth base, Miller′s modified

Tryptone, yeast extract and sodium chloride

Synonyme(s) :

Lennox broth base, modified, Luria-Bertani broth base, modified, Lysogeny broth base, modified

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A propos de cet article

NACRES:
NA.72
UNSPSC Code:
41106200
Service technique
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Quality Segment

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
microbiology

General description

Luria broth (LB) is a nutritionally rich bacterial culture medium that is commonly used for maintaining and cultivating laboratory recombinant strains of E. coli. It has potential applications in molecular microbiology methods such as the preparation of plasmid DNA and recombinant proteins. Luria broth base, Miller′s modified is used to culture members of the family Enterobacteriaceae and for coliphage plaque assays.

Application

Luria broth base, Miller′s modified has been used as a nutrient culture medium for E-coli cells.

Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).

Preparation Note

Recommended use at 15.5 g per liter.

Other Notes

Contains: Tryptone, yeast extract and sodium chloride.


Classe de stockage

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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