L2625
L-Lactic Dehydrogenase from bovine heart
Type III, ammonium sulfate suspension, ≥500 units/mg protein
Synonyme(s) :
Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
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About This Item
Produits recommandés
Type
Type III
Forme
ammonium sulfate suspension
Activité spécifique
≥500 units/mg protein
Activité étrangère
pyruvate kinase ≤0.03%
Température de stockage
2-8°C
Informations sur le gène
cow ... LDHA(281274) , LDHB(281275)
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Description générale
Research area: Cell signaling
Lactate dehydrogenase (LDH) is a cytoplasmic enzyme that belongs to the oxidoreductase’s family. It is ubiquitously expressed in body tissues but at high levels in muscle, liver, and kidney. LDH exists as tetramer and comprises of two subunits (H and M). The LDH of eukaryotes undergo active-site loop gating for their catalytic functionality. L-lactate, which is the major enantiomeric form of lactate in vertebrates, is produced by the L-isomers of LDH enzyme encoded by the LDHC gene.
Lactate dehydrogenase (LDH) is a cytoplasmic enzyme that belongs to the oxidoreductase’s family. It is ubiquitously expressed in body tissues but at high levels in muscle, liver, and kidney. LDH exists as tetramer and comprises of two subunits (H and M). The LDH of eukaryotes undergo active-site loop gating for their catalytic functionality. L-lactate, which is the major enantiomeric form of lactate in vertebrates, is produced by the L-isomers of LDH enzyme encoded by the LDHC gene.
Application
L-Lactic Dehydrogenase from bovine heart has been used as a standard in cytotoxicity assay. It has also been used in glutamic pyruvic transaminase (GPT) assay.
Actions biochimiques/physiologiques
Lactate dehydrogenase (LDH) is a crucial enzyme that plays a vital role in the anaerobic metabolic pathway. It facilitates the conversion of lactate to pyruvate and NAD⁺ to NADH, and vice versa, within cells. This enzymatic reaction plays a crucial role in cellular energy production, particularly in environments with low oxygen levels or hypoxic conditions. LDH activity is also significant in tumor glycolysis and the Warburg effect. LDH is released into the bloodstream when the cells are damaged or undergo death. Therefore, LDH can be used as a potential marker of cell injury and death. High lactate dehydrogenase activity is associated with various medical conditions, such as liver disease, anemia, heart attack, bone fractures, muscle trauma, and certain types of cancer.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Définition de l'unité
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
Forme physique
Crystalline suspension in 2.1 M (NH4)2SO4 solution, pH 6.0
Remarque sur l'analyse
Protein determined by biuret
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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