I2771
ITS+3 Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonyme(s) :
ITS Supplement
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About This Item
Code UNSPSC :
12352205
Nomenclature NACRES :
NA.75
Produits recommandés
Stérilité
sterile-filtered
Gamme de produits
BioReagent
Forme
liquid
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Conditions d'expédition
ambient
Température de stockage
2-8°C
Description générale
ITS+3 liquid media supplement is a mixture of insulin, human transferrin (partially iron-saturated), sodium selenite, linoleic acid, and bovine serum albumin (BSA). It is suitable for serum-free cell culture applications.
Application
ITS+3 Liquid Media Supplement (100×) has been used as a media supplement:
- to support the growth of human adipose stem cells (ASC)
- to culture porcine intervertebral disc (IVD) cells and tissues
- to culture cartilage endplate (CEP) cells
Actions biochimiques/physiologiques
- Insulin: a polypeptide hormone that promotes glucose and amino acid uptake and may produce a mitogenic effect.
- Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.
- Selenium: an essential trace element present in serum.
Composants
Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 470 μg/ml linoleic acid, 470 μg/ml oleic acid and 50 mg/ml bovine serum albumin at the 100x concentration. Contains 2 moles each of linoleic acid and oleic acid per mole of albumin.
Notes préparatoires
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
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