G7654
Gel Loading Solution
for NA electrophoresis, solution
Synonyme(s) :
DNA Gel Loading Solution, Gel Loading Buffer
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About This Item
Code UNSPSC :
12161703
Nomenclature NACRES :
NA.25
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Forme
solution
Concentration
6 ×
Température de stockage
2-8°C
Description générale
Gel loading solution is used as a tracking dye during electrophoresis. The dyes have a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:6 with sample before loading.
Application
Suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.
Composants
Gel loading buffer contains 0.25% bromophenol blue, 0.25% xylene cyanol, and 40% sucrose.
Autres remarques
Band migration can be expected as follows:
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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