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G5507

Sigma-Aldrich

Galactosyltransferase from bovine milk

lyophilized powder

Synonyme(s) :

Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase

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About This Item

Numéro CAS:
9031-68-9
Numéro de classification (Commission des enzymes):
2.4.1.22 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131122
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Niveau de qualité

200

Activité spécifique

3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)

Composition

Protein, 35-65% modified Warburg-Christian

Impuretés

≤10 % protein α-lactalbumin

Température de stockage

−20°C

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Description générale

Research area: Cell signalling. Galactosyltransferase(GalT) is found in both prokaryotes and eukaryotes.Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.

Application

Galactosyltransferase from bovine milk has been used:
  • in the generation of hyper-galactosylated variant of IgG1
  • in β -1,4-galactosyltransferase assay rice root cell extracts
  • in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Actions biochimiques/physiologiques

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.
Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various disease states.Galactosyltransferase(GalT) functions either as a soluble enzyme or as membrane-bound moieties.

Liaison

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Définition de l'unité

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Forme physique

Lyophilized powder containing Tris, EDTA, and (NH4)2SO4

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

The kinetic mechansim of bovine milk galactosyltransferase. The role of alpha-lactalbumin.
Bell JE, et al.
The Journal of Biological Chemistry, 251(10), 3003-3013 (1976)
A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities.
J A Campbell et al.
The Biochemical journal, 326 ( Pt 3), 929-939 (1997-10-23)
A novel short-root gene encodes a glucosamine-6-phosphate acetyltransferase required for maintaining normal root cell shape in rice
Jiang H, et al.
Plant Physiology, 138(1), 232-242 (2005)
Structure and function of beta-1, 4-galactosyltransferase
Qasba PK, et al.
Current Drug Targets, 9(4), 292-309 (2008)
The role of alpha-lactalbumin and the A protein in lactose synthetase: a unique mechanism for the control of a biological reaction.
K Brew et al.
Proceedings of the National Academy of Sciences of the United States of America, 59(2), 491-497 (1968-02-01)
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