D4309
REDTaq® DNA Polymerase
Taq for routine PCR with inert dye, 10X buffer included
Synonyme(s) :
DNA polymerase with loading dye, Taq DNA polymerase
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Niveau de qualité
Forme
liquid
Utilisation
sufficient for 1000 reactions
sufficient for 250 reactions
sufficient for 50 reactions
Caractéristiques
dNTPs included: no
hotstart: no
Concentration
1 unit/μL
Technique(s)
PCR: suitable
Couleur
red
Entrée
purified DNA
Adéquation
suitable for PCR
Application(s)
agriculture
Conditions d'expédition
wet ice
Température de stockage
−20°C
Description générale
REDTaq® DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.
Application
For routine PCR amplifications REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis.
Caractéristiques et avantages
- Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications.
- Visual confirmation that not only has the enzyme been added, but that proper mixing has occurred.
- No additional loading dyes are necessary. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis.
Conditionnement
Provided with 10X reaction buffer containing MgCl2
Définition de l'unité
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
Informations légales
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
En option
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Mutation or polymorphism of the CD20 gene is not associated with the response to R-CHOP in diffuse large B cell lymphoma patients
Sar A, et al.
Leukemia Research, 33(6), 792-797 (2009)
Mapping networks of protein-mediated physical interactions between chromitin elements.
Tiwari, V.K., and Baylin, S.B.
Current Protocols in Molecular Biology, 21-21 (2010)
Proteomics-based approach to elucidate the mechanism of antitumor effect of curcumin in cervical cancer
Madden K, et al.
Prostaglandins, Leukotrienes, and Essential Fatty Acids, 80(1), 9-18 (2009)
Innis, M.A., and Gelfand, D.H.
PCR Protocols: A Guide to Methods and Applications, 3-3 (1990)
Ovidiu Paun et al.
Methods in molecular biology (Clifton, N.J.), 862, 75-87 (2012-03-16)
Amplified fragment length polymorphism (AFLP) is a PCR-based technique that uses selective amplification of a subset of digested DNA fragments to generate and compare unique fingerprints for genomes of interest. The power of this method relies mainly in that it
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique