C0678
Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse
clone VC1.1, ascites fluid, buffered aqueous solution
Synonyme(s) :
Monoclonal Anti-HNK-1, Anti-CD57, Anti-N-CAM
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
VC1.1, monoclonal
Forme
buffered aqueous solution
Contient
15 mM sodium azide
Espèces réactives
mouse, rat, feline, human
Technique(s)
western blot: 1:4,000 using cerebral cortex extract
Isotype
IgM
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... B3GAT1(27087)
mouse ... B3gat1(76898)
rat ... B3gat1(117108)
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Description générale
Monoclonal Anti-HNK-1/N-CAM (CD57) (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Human natural killer cell -1 (HNK-1), also referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP, is mapped to human chromosome 11q25. The encoded protein belongs to the glucuronyltransferase gene family. Neural cell-adhesion molecule (N-CAM) is expressed on all neuroepithelial cells from the time of neural induction in the early embryo. It is known that the sulfated carbohydrate HNK-1 epitope is carried by several surface molecules including a proportion of N-CAM isoforms.
Spécificité
Recognizes the CD57/HNK-1 human myeloid cell associated surface glycoprotein. The epitope recognized is an N-linked carbohydrate which is present in a variety of glycoproteins and in some glycolipids. It is resistant to formalin fixation and paraffin embedding. VC1.1 antibody and the HNK-1 (Leu7) antibody inhibit the binding of each other. The antibody recognizes myelin associated glycoprotein in some species and a high molecular weight chondroitin sulphate proteoglycan.
5th Workshop: code no. NK 67
5th Workshop: code no. NK 67
Immunogène
A homogenate of cat primary visual cortex (area 17)
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used:
- as primary antibody for western blot analysis
- immunocytochemistry
- immunocytochemical
- immunohistological staining
Actions biochimiques/physiologiques
HNK1 (human natural killer-1) is referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP. It is a member of the glucuronyltransferase gene family. Human natural killer-1 (HNK1) carbohydrate epitope is expressed on various cell adhesion molecules in the nervous system and plays a role in cell-cell and cell-substrate interactions. HNK-1 epitope stabilizes GluR2 on neuronal surface membranes and regulates cell surface stability of GluR2 by modulating the interaction with N-cadherin. It is also plays a role in cell migration and synaptic plasticity. HNK-1 epitope has been associated with the risk of metastasis. It is observed to be highly expressed in melanoma cell lines.
Description de la cible
CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, about 60% of NK active cells and on a subset of T cells.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Ronald S Goldstein
Methods in molecular biology (Clifton, N.J.), 331, 137-151 (2006-08-03)
The traditional methods of studying the differentiation of human embryonic stem cells (hESCs) are to differentiate them in vitro or in immune-deficient mice as teratomas. The chick embryo is a well-studied and accessible experimental system that has been shown to
Jean-Marie Gasc et al.
Biology open, 4(5), 666-671 (2015-04-04)
The enteric nervous system originates from neural crest cells that migrate in chains as they colonize the embryonic gut, eventually forming the myenteric and submucosal plexus. Failure of the neural crest cells to colonize the gut leads to aganglionosis in
Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid: inhibitory effect of serum
Pachernik J, et al.
Physiological Research, 54(1), 115-122 (2005)
Yi-Sen Cheng et al.
Anatomical record (Hoboken, N.J. : 2007), 298(7), 1271-1281 (2015-03-27)
Sorafenib has been used as an oral anti-cancer drug because of its ability to inhibit tumor growth. However, the pharmacological effect of sorafenib is still the lack of in vivo experimental evidence. Tumor and embryonic cells share some similar features
Xiao-Tan Zhang et al.
Zygote (Cambridge, England), 26(6), 457-464 (2018-12-07)
SummaryFibroblast growth factor (FGF) signalling acts as one of modulators that control neural crest cell (NCC) migration, but how this is achieved is still unclear. In this study, we investigated the effects of FGF signalling on NCC migration by blocking
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique