Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

C6680

Sigma-Aldrich

Anti-HNK-1/N-CAM (CD57) antibody, Mouse monoclonal

clone VC1.1, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-HNK-1, Hnk-1 Antibody, Hnk-1 Antibody - Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse, Anti-CD57, Anti-N-CAM

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

VC1.1, monoclonal

Forme

buffered aqueous solution

Espèces réactives

mouse, rat, feline, human

Technique(s)

flow cytometry: 5 μL using 1 × 106 cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

Isotype

IgM

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Mouse Monoclonal Anti-Human CD57/HNK-1 (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a homogenate of cat cerebral cortical area 17. Human natural killer-1 (HNK-1) also called β-1,3-glucuronyltransferase (B3GAT1), is mapped to human chromosome 11q25 and belongs to the glucuronyltransferase gene family. CD57/ HNK-1 is expressed on a subpopulation (15-20%) of peripheral blood mononuclear cells. Expression of the antigen was described for about 60% of NK active cells and for a T-cell subset.

Spécificité

Recognizes the CD57/HNK-1 human myeloid cell associated surface glycoprotein. The epitope recognized is an N-linked carbohydrate which is present in a variety of glycoproteins and in some glycolipids. It is resistant to formalin fixation and paraffin embedding. VC1.1 antibody and the HNK-1 (Leu7) antibody inhibit the binding of each other. The antibody recognizes myelin associated glycoprotein in some species and a high molecular weight chondroitin sulphate proteoglycan.
5th Workshop: code no. NK 67

Immunogène

A homogenate of cat primary visual cortex (area 17)

Application

Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used in:
  • immunohistochemistry
  • immunostaining and microscopy
  • immunocytochemistry
  • immunofluorescence

Actions biochimiques/physiologiques

Human natural killer-1 (HNK-1)/cluster of differentiation (CD57) is also found on some B-cell lines and B lymphocytic leukemia (B-CLL) melanoma lines. The antigen is also detected in lymphoid tissues, certain neuronal subsets, peripheral nerve Schwann cells, and normal, benign, and malignant prostate. Neurofibromas, schwannomas, traumatic neuromassmall cell lung carcinomas frequently express HNK-1. CD57/HNK-1 appears to be expressed in many species ranging from insects to humans. It is thought to be involved in cellular adhesion events and is expressed on several adhesion related proteins including most neural immunoglobulins superfamily molecules.

Description de la cible

CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, about 60% of NK active cells and on a subset of T cells.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

A robust method to derive functional neural crest cells from human pluripotent stem cells
Kreitzer FR, et al.
American Journal of Stem Cells, 2(2), 119-119 (2013)
A secretory cell type develops alongside multiciliated cells, ionocytes and goblet cells, and provides a protective, anti-infective function in the frog embryonic mucociliary epidermis
Dubaissi E, et al.
Development, 141(7), 1514-1525 (2014)
Immunohistological analysis of CD1a+ langerhans cells and CD57+ natural killer cells in healthy and diseased human gingival tissue: A comparative study
Stelin S, et al.
Journal of Indian Society of Periodontology, 13(3), 150-150 (2009)
Michelle Ware et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 244(10), 1202-1214 (2015-08-01)
The early axon scaffold is the first axonal structure to appear in the rostral brain of vertebrates, paving the way for later, more complex connections. Several early axon scaffold components are conserved between all vertebrates; most notably two main ventral
Richard W Naylor et al.
PloS one, 11(10), e0165464-e0165464 (2016-10-30)
Corneal diseases such as keratoconus represent a relatively common disorder in the human population. However, treatment is restricted to corneal transplantation, which only occurs in the most advanced cases. Cell based therapies may offer an alternative approach given that the

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique