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B3679

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

Synonyme(s) :

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.83

product name

BCIP®/NBT-Purple Liquid Substrate System for Membranes, ready to use alkaline phosphatase substrate

Niveau de qualité

100

Forme

liquid

Température de stockage

2-8°C

Catégories apparentées

Application

BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
  • for the detection of secondary antibodies by immunoblot
  • in ELISPOT assay
  • in dot blot analysis
  • in western blot to visualize protein bands on nitrocellulose membrane

Actions biochimiques/physiologiques

5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.

Attention

Stable at least one year at 2-8 °C.

Forme physique

Ready-to-use.

Informations légales

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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