B6149

5-Bromo-4-chloro-3-indolyl phosphate disodium salt

chromogenic, ≥98% (HPLC), powder

• Synonyme(s) : 5-BCIP disodium salt, BCIP^®, X-phosphate disodium salt
• Formule linéaire : C₈H₄BrClNO₄P · 2Na
• Numéro CAS: 102185-33-1
• Poids moléculaire : 370.43
• Numéro MDL: MFCD00036757
• Code UNSPSC : 12352204
• ID de substance PubChem : 24891897
• Nomenclature NACRES : NA.83

Propriétés

• product name: 5-Bromo-4-chloro-3-indolyl phosphate disodium salt, ≥98% (HPLC)
• Pureté: ≥98% (HPLC)
• Forme: powder
• Solubilité: H₂O: 20 mg/mL; DMF: insoluble
• Température de stockage: −20°C
• Chaîne SMILES: [Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12
• InChI: 1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
• Clé InChI: OAZUOCJOEUNDEK-UHFFFAOYSA-L

Description

Application

5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Substrats

Histochemical substrate for alkaline phosphatase.

Informations légales

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)