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A2237

Sigma-Aldrich

Phosphatase, Alkaline shrimp

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

Synonyme(s) :

Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

Numéro CAS:
9001-78-9
Numéro de classification (Commission des enzymes):
3.1.3.1 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131849
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Produit recombinant

expressed in proprietary host

Niveau de qualité

200

Forme

buffered aqueous glycerol solution

Concentration

≥900 DEA units/mL

Activité étrangère

RNAse, DNAse, none detected

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

Application

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.

Actions biochimiques/physiologiques

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

Définition de l'unité

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

Forme physique

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2

Remarque sur l'analyse

Protein determined by biuret

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Nicoletta I Petridou et al.
Cell, 184(7), 1914-1928 (2021-03-18)
Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural
Lars Hansen et al.
American journal of human genetics, 92(4), 575-583 (2013-04-09)
PGAP2 encodes a protein involved in remodeling the glycosylphosphatidylinositol (GPI) anchor in the Golgi apparatus. After synthesis in the endoplasmic reticulum (ER), GPI anchors are transferred to the proteins and are remodeled while transported through the Golgi to the cell
Insulin affects dopamine metabolism in Drosophila females under normal and stress conditions.
N V Adonyeva et al.
Doklady. Biochemistry and biophysics, 448, 40-42 (2013-03-13)
Yuan-Kun Zhai et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 38(1), 105-111 (2013-04-20)
To investigate the effects of naringin on the proliferation, differention and maturaion of rat calvarial osteoblasts (ROB). Segregated neonatal SD rat skull, enzyme digestion to obtain ROB. The culture medium was replaced every three days. Serial subcultivation proceeded when cells
Interpreting an isolated raised serum alkaline phosphatase level in an asymptomatic patient.
Kate Elizabeth Shipman et al.
BMJ (Clinical research ed.), 346, f976-f976 (2013-04-05)
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