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A1009

Sigma-Aldrich

Albumin methylated from bovine serum

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About This Item

Numéro MDL:
MFCD00145745
Code UNSPSC :
12352202
eCl@ss :
42010201
Nomenclature NACRES :
NA.26

Source biologique

bovine

Niveau de qualité

200

Forme

powder

Origine

USA origin

Technique(s)

affinity chromatography: suitable
fractionation: suitable

Impuretés

≤10% water (Karl Fischer)

Numéro d'accès UniProt

P02769

Température de stockage

2-8°C

Informations sur le gène

bovine ... ALB(280717)

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Application

Albumin methylated from bovine serum (mBSA) has been used as a coating for immunoplates in enzyme-linked immunosorbent assay (ELISA) of anti-dsDNA IgG antibodies. It has also been used to induce arthritis in mice models.
As an affinity ligand on diatomaceous earth support (MAK chromatography), a medium for fractionation of different forms of DNA and for separating aminoacyl-tRNAs. Different modes of binding of double-stranded, denatured, and replicating forms of DNA account for the efficacy of the method.

Actions biochimiques/physiologiques

Albumin is a major circulating component of the plasma and is a highly soluble multifunctional protein. It plays a role in several physiological and pharmacological processes. Albumin binds to metals, cholesterol, hormones, bile pigments, drugs, and fatty acids to facilitate their transport. It regulates osmotic pressure and distributes fluids between various compartments.

Notes préparatoires

Methyl ester of albumin prepared per Mandell, J.D. and Hershey, A.D., Anal. Biochem., 1, 66 (1960).

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Albumine de sérum bovin chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%

Sigma-Aldrich

A1933

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Albumine de sérum bovin lyophilized powder, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A2153

Albumine de sérum bovin

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Fractionation of amino acyl-acceptor RNA on a methylated albumin column.
N SUEOKA et al.
Proceedings of the National Academy of Sciences of the United States of America, 48, 1454-1461 (1962-08-01)
Fractionation of nucleic acids with the methylated albumin column.
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Journal of molecular biology, 4, 161-172 (1962-03-01)
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