48724

Gelatin from porcine skin

suitable for microbiology, high gel strength

• Numéro CAS: 9000-70-8
• Numéro CE : 232-554-6
• Numéro MDL: MFCD00081638
• Code UNSPSC : 41106212
• Nomenclature NACRES : NA.85

Propriétés

• Source biologique: Porcine
• Niveau de qualité: 100
• Stérilité: non-sterile
• Forme: powder
• Qualité: high gel strength
• Durée de conservation: limited shelf life, expiry date on the label
• Résidus de calcination: ≤2%
• Perte: ≤15% loss on drying
• Transmittance: 450 nm, ≥85%; 620 nm, ≥95%
• pH: 4.0-6.0 (25 °C, 67 mg/mL in H₂O); 5.20-5.60
• Viscosité: 5.10-5.80 mPa.s
• Résistance du gel: 245-275 g Bloom (67 mg/ml water); 280-320 g BloomAOAC
• Solubilité: H₂O: 67 mg/mL at 50 °C, slightly hazy, faintly yellow; H₂O: 67 mg/mL at 60 °C
• Traces d'anions: chloride (Cl^-): ≤2000 mg/kg
• Traces de cations: Ca: ≤2000 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤10 mg/kg; Cu: ≤50 mg/kg; Fe: ≤50 mg/kg; K: ≤500 mg/kg; Mg: ≤500 mg/kg; Mn: ≤5 mg/kg; Na: ≤1200 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤10 mg/kg
• Application(s): microbiology

Description

Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm² or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Composants

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Attention

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Notes préparatoires

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 250. Manufactured by Gelita AG

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)