48722

Gelatin from porcine skin

medium gel strength, suitable for microbiology

• Synonyme(s) : Gelatine, hydrolyzed collagen, collagen hydrolysate, gelatine hydrolysate
• Numéro CAS: 9000-70-8
• Numéro CE : 232-554-6
• Numéro MDL: MFCD00081638
• Code UNSPSC : 41106212
• Nomenclature NACRES : NA.85

Propriétés

• Source biologique: Porcine
• Niveau de qualité: 200
• Stérilité: non-sterile
• Forme: powder
• Qualité: medium gel strength
• Conditionnement: bottle of 100 g; bottle of 500 g
• Résidus de calcination: ≤2%
• Perte: ≤15% loss on drying
• Couleur: light yellow
• pH: 4.0-6.0 (25 °C, 67 mg/mL in H₂O)
• Résistance du gel: 165-195 g Bloom (67 mg/ml water)
• Solubilité: H₂O: 67 mg/mL at 50 °C, slightly hazy, slightly yellow
• Traces d'anions: chloride (Cl^-): ≤3000 mg/kg
• Traces de cations: Ca: ≤2000 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤10 mg/kg; Cu: ≤50 mg/kg; Fe: ≤50 mg/kg; K: ≤500 mg/kg; Mg: ≤500 mg/kg; Mn: ≤5 mg/kg; Na: ≤5000 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤10 mg/kg
• Application(s): microbiology

Description

Description générale

Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred to as type A. It is a hydrocolloid and is rich in glycine, proline, and hydroxyproline, which impart structural stability. It is synthesized from the alkaline digestion of collagen from porcine. Gelatin takes up a random coil structure after digestion from the triple helical collagen.

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc., in water. Type A gelatin is derived from acid-cured tissue, and Type B from lime-cured tissue.

The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 180.

Application

Gelatin has been used in many applications, such as in coating cell culture plates to improve attachment of cells, in PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology.

Composants

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Attention

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Notes préparatoires

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. Manufactured by Gelita AG

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): nwg
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)