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11299964001

Roche

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II

sufficient for ≤100 tests, storage temp.:-20°C

Synonyme(s) :

5-BrdU, 5-Bromo-2-deoxyuridine

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About This Item

Code UNSPSC :
41116133

Niveau de qualité

100

Utilisation

sufficient for ≤100 tests

Fabricant/nom de marque

Roche

Température de stockage

−20°C

Description générale

Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [ 3H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2′-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Immunohistocytochemical assay for the detection of 5-bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA.

Spécificité

Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.

Application

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II has been used in:
  • labeling of tooth roots for histology
  • immunostaining of mice frontal sections
  • immunofluorescence imaging of hepatocellular carcinoma sections

The kit is used for the detection of BrdU incorporated into cellular DNA by immunohistocytochemistry.

Caractéristiques et avantages

  • Safe: No radioisotopes are used.
  • Easy to perform: Follows a standard immunohistochemistry protocol.
  • Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU.
  • Flexible: Allows double-labeling protocols.

Conditionnement

1 kit containing 7 components.

Principe

Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU, which contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments that allow binding of the antibody to BrdU. Next, an alkaline phosphatase (AP)-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. The sample is then incubated with the AP substrate and NBT/ BCIP, which is metabolized to form a colored reaction product. The sample is evaluated using a phase-contrast microscope.

Notes préparatoires

Working concentration: Working concentration of the labeling reagent corresponds to the WC of the In Situ Cell Proliferation Kits.
Sample material:
Cell culture: adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling).

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description
  • BrdU Labeling Reagent 1,000x concentrated
  • Washing Buffer concentrate 10x concentrated
  • Incubation Buffer
  • Anti-BrdU antibody, contains nucleases for DNA denaturation
  • Anti-mouse Ig-alkaline Phosphatase antibody
  • NBT
  • BCIP

Pictogrammes

FlameExclamation markHealth hazard
GHS02,GHS07,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 Dermal - Muta. 1B - Repr. 1B - Skin Sens. 1

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

136.4 °F

Point d'éclair (°C)

58 °C

Certificats d'analyse (COA)

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