11089153001
Roche
Streptavidin-POD conjugate
liquid, pkg of 1 mL
Synonyme(s) :
streptavidin
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About This Item
Code UNSPSC :
12352200
Produits recommandés
Forme
liquid
Niveau de qualité
Conditionnement
pkg of 1 mL
Fabricant/nom de marque
Roche
Température de stockage
-10 to -25°C
Description générale
Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.
Application
Streptavidin-POD has been used for:
- ELISA
- In situ hybridization
- Western blot
Définition de l'unité
One unit Streptavidin-POD, corresponding to one unit peroxidase, will oxidize 1 μmol of guaiacol tetramer in one minute at +25 °C and pH 7.0.
Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).
Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).
Forme physique
Liquid, stabilized
Notes préparatoires
Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted).Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
- ELISA: 50 to 200 mU/ml
- Immunohistocytochemistry: 250 to 1000 mU/ml
- Western blot: 200 to 500 mU/ml
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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