10837059001
Roche
Lysozyme
from hen egg white
Synonyme(s) :
Mucopeptide N-acetylmuramoyl-hydrolase, Muramidase, lysozyme
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About This Item
Produits recommandés
Source biologique
chicken egg white
Niveau de qualité
Pureté
≥95%
Forme
solid
Activité spécifique
>23000 U/mg (Shugar units)
Poids mol.
14.4 kDa
Conditionnement
pkg of 10 g
Fabricant/nom de marque
Roche
pH optimal
6.0-7.0
Absorption
3.9 at 280 nm (10 mg enzyme/ml)
Description générale
Muramidase, Mucopeptide N-acetylmuramoyl-hydrolase
Application
Lysozyme has been used for:
- Cell wall degradation
- Preparation of protoplasts
- Bacteriolysis
- Pharmacology (anti-inflammatory effect on mucous membrane; healing of tissue; protection against bleeding; normalization of mucus secretion; dissolution of pyrogens; potentiation of antibiotics)
- Food and drinks (flavor enhancer)
- Sample preparation prior to isolation of nucleic acids
Lysozyme has been used in the monitoring of transcription by real-time -PCR and sample preparation.
Actions biochimiques/physiologiques
Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.
Définition de l'unité
One Shugar unit is that quantity of enzyme in 1 ml of a suspension of Micrococcus luteus of pH 7.0 whose initial absorbance at a wavelength of 450 nm is 0.750 for a pathlength of 10 mm which causes the absorbance to decrease at the rate of 0.001 per minute, all measurements being carried out at a temperature of +25 °C.
Notes préparatoires
Working solution: Lysozyme hydrochloride is readily soluble in water and buffer solutions but insoluble in organic solvents.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.
Reconstitution
Prepare a stock solution at a concentration of 50 mg/ml in dist. water. Dispense into aliquots and store at -15 to -25 °C. Discard each aliquot after use.
Stockage et stabilité
Store at 2 to 8 °C. (Store dry!)
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
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