MAB10754

Anti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1

clone 2B12.1, from mouse

• Synonyme(s) : caspase 8, apoptosis-related cysteine peptidase, caspase-8, caspase 8, apoptosis-related cysteine protease, MACH-beta-1/2/3/4 protein, Apoptotic protease Mch-5, Apoptotic cysteine protease, MACH-alpha-1/2/3 protein, FADD-homologous ICE/ced-3-like proteas
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 2B12.1, monoclonal
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgMκ
• Numéro d'accès NCBI: NP_001073593
• Numéro d'accès UniProt: Q14790
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CASP8(841)

Description

Description générale

Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death. Caspase-8 is recruited to death-inducing signaling complex (DISC), CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18. Active caspase-8 or the p18 subunit, is the first in the caspase signaling cascade, and it signals downstream caspases to eventually induce apoptosis.

Spécificité

This antibody recognizes the active form (p18 subunit) of Caspase-8.

Immunogène

KLH-conjugated linear peptide corresponding to the active form of human Caspase-8.

Application

Detect Caspase-8 (active form p18 subunit) using this Anti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1 validated for use in WB & IC.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected active Caspase-8 (p18 subunit) in etoposide-treated HeLa cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Caspases

Apoptosis - Additional

Qualité

Evaluated by Western Blot in camptothecin-treated and untreated Jurkat lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected the active form of Caspase-8 (p18 subunit) in 10 µg of camptothecin-treated Jurkat lysates. Untreated jurkat lysate exhibited no detection of active Caspase-8.

Description de la cible

~18 kDa observed

Liaison

Replaces: 04-573; 04-574

Forme physique

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Camptothecin-treated and untreated Jurkat lysates

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable