Accéder au contenu
Merck
Toutes les photos(1)

Documents

324355

Millipore

n-Dodecyl β-D-maltoside

ULTROL® Grade, ≥98% (HPLC), non-ionic detergent for the stabilization and activation of enzymes

Synonyme(s) :

n-Dodecyl-β-D-maltoside, ULTROL® Grade, DDM, Dodecyl maltoside, Lauryl-β-D-maltoside

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12161902
Nomenclature NACRES :
NA.25

Niveau de qualité

Pureté

≥98% (HPLC)

Forme

solid

Poids mol.

micellar wt 50000

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
desiccated (hygroscopic)

Nombre d'agrégation

98

Impuretés

≤1% dodecanol 1H NMR

Couleur

white

CMC

100-600 μM

Solubilité

water: 100 mg/mL

Conditions d'expédition

ambient

Température de stockage

2-8°C

InChI

1S/C24H46O11/c1-2-3-4-5-6-7-8-9-10-11-12-32-23-21(31)19(29)22(16(14-26)34-23)35-24-20(30)18(28)17(27)15(13-25)33-24/h15-31H,2-14H2,1H3/t15-,16-,17-,18+,19-,20-,21-,22-,23-,24-/m1/s1

Clé InChI

NLEBIOOXCVAHBD-QKMCSOCLSA-N

Description générale

A water-soluble nonionic detergent. Studies on rhodopsin have shown that alkyl glycosides with longer alkyl chains are better at preserving the protein′s properties. In addition, dodecyl maltoside′s longer hydrophilic group confers higher solubility in water. This is in contrast to undecyl and dodecyl glucosides, which have low solubility in water. Due to its low CMC, dodecyl maltoside is not easily removed by dialysis. The half time for dialysis is 20 h. This detergent is milder than nonyl glucoside. Of several glycosides tested, dodecyl maltoside was found to be the most useful for the preparation of active cytochrome oxidase from Neurospora. In octyl glucoside, cytochrome oxidase dissociated into its subunits and became inactive. Similarly, beef heart oxidase when equilibrated in octyl glucoside formed a mixture of polymer, dimer, and lower molecular weight forms and underwent denaturation. In contrast, the enzyme, when equilibrated in dodecyl maltoside, was found to exist as a uniform species of 30 kDa, which is consistent with an active dimeric form. Dodecyl maltoside has a critical micelle concentration (CMC) of 100-600 µM and a micellar weight of 70,000. Aggregation number: 98.
Absorbance (10%, H2O, 260 nm): <1.0.
Non-ionic detergent similar to n-octyl-β-D-glucopyranoside; however, increased alkyl chain length has been shown to increase thermal stability of lipid-free rhodopsin in solution. Useful in membrane-protein solubilization studies. Aggregation number: 98.

Application


  • Maximizing hydrophobic peptide recovery in proteomics and antibody development using a mass spectrometry compatible surfactant.: The article explores the use of n-Dodecyl β-D-maltoside to maximize hydrophobic peptide recovery in proteomics. The findings highlight the surfactant′s compatibility with mass spectrometry, improving peptide analysis and antibody development. (Nie et al., 2022).

  • Sickle-like Inertial Microfluidic System for Online Rare Cell Separation and Tandem Label-Free Quantitative Proteomics (Orcs-Proteomics).: This research introduces an inertial microfluidic system utilizing n-Dodecyl β-D-maltoside for rare cell separation and proteomics. The study underscores the surfactant′s role in enhancing cell recovery and proteomic analysis. (Wang et al., 2022).

  • Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics.: This paper presents a method for single-cell proteomics using n-Dodecyl β-D-maltoside. The surfactant assists in efficient sample preparation, facilitating high-resolution proteomic analysis. (Tsai et al., 2021).


Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, refrigerate (4°C).

Stock solutions are stable for up to 3 months at 4°C.

Autres remarques

Witzmann, F., et al. 1991. Electrophoresis12, 687.
Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem.171, 330.
Rosevear, P., et al. 1980. Biochemistry19, 4108.
Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids23, 321.
Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta425, 46.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 2

1 of 2

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique