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Key Documents

SRP3135

Sigma-Aldrich

p16-INK-4a TAT

recombinant, expressed in E. coli, lyophilized, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for mammalian cell culture

Synonym(s):

CDK4I, Cyclin-dependent kinase 4 inhibitor A, Cyclin-dependent kinase inhibitor 2A, Multiple tumor suppressor 1, p16-INK4, p16INK4A

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

product name

p16-INK-4a TAT human, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

biological source

human

recombinant

expressed in E. coli

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

18 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

P42771

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CDKN2A(1029)

General description

Cyclin dependent kinase inhibitor 2A (CDKN2A) also known as p16-INK-4a, belongs to the Ink4 (inhibitor of CDK4) family of CDK inhibitors. It is encoded by the gene mapped to human chromosome 9p21 within the INK4a/ alternate reading frame (ARF) locus. Human P16-INK4A is localized in both nucleus and cytoplasm, and its expression increases with ageing in skin and kidney tissues.

Biochem/physiol Actions

Cyclin dependent kinase inhibitor 2A (CDKN2A), also known as p16-INK-4a, arrests cell cycle progression by inhibiting S-phase and promotes apoptosis. The encoded protein has anti-proliferative biological activity, and is implicated in the retinoblastoma (Rb) protein pathway. p16-INK-4a acts as a tumor suppressor and plays a vital role in ageing and cellular senescence. Increased expression of this protein might lead to various types of high grade cancers. p16-INK-4a also facilitates cell invasion and angiogenesis. Bronchial lavage P16INK4A promoter methylation might function as a potential biomarker for diagnosis of lung cancer.

Sequence

EPAAGSSMEP SADWLATAAA RGRVEEVRAL LEAGALPNAP NSYGRRPIQV MMMGSARVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLVVLHRAG ARLDVRDAWG RLPVDLAEEL GHRDVARYLR AAAGGTRGSN HARIDAAEGP SDIPDGYGR KKRRQRRR

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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p16(Ink4a) overexpression in cancer: a tumor suppressor gene associated with senescence and high-grade tumors.
Oncogene (2011)
Antiproliferative effects of local anesthetics on mesenchymal stem cells: potential implications for tumor spreading and wound healing.
Lucchinett E
Anesthesiology (2012)
Expression and significance of p53, rb, p21/waf-1, p16/ink-4a, and PTEN tumor suppressors in canine melanoma.
Koenig A
Veterinary Pathology (2002)
Jinsuke Nishino et al.
Cell, 135(2), 227-239 (2008-10-30)
Stem cells persist throughout life in diverse tissues by undergoing self-renewing divisions. Self-renewal capacity declines with age, partly because of increasing expression of the tumor suppressor p16(Ink4a). We discovered that the Hmga2 transcriptional regulator is highly expressed in fetal neural
A Okamoto et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(23), 11045-11049 (1994-11-08)
Cell cycle arrest at the G1 checkpoint allows completion of critical macromolecular events prior to S phase. Regulators of the G1 checkpoint include an inhibitor of cyclin-dependent kinase, p16INK4; two tumor-suppressor proteins, p53 and RB (the product of the retinoblastoma-susceptibility

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