SEQXI
SEQPLEX-I WGA Kit
Whole Genome Amplification, DNA Amplification
Synonym(s):
SeqX-i DNA Amplification kit, The SeqPlex™-I DNA Amplification Kit for whole genome amplification (WGA), WGA Kit, Whole Genome Amplification kit
About This Item
Recommended Products
technique(s)
DNA amplification: suitable
PCR: suitable
whole genome amplification: suitable
compatibility
Illumina Next Generation Sequencing
shipped in
wet ice
storage temp.
−20°C
General description
Application
Features and Benefits
- Amplifies fragmented/extremely small quantities of DNA: Yields from ChIP or FFPE, which are 200-500 bp are easily amplified by Random priming technology.
- Facilitates sequencing from as little as 100 pg of DNA
- Enhanced primers for complete genome coverage, minimal sequence bias, and amplicon size ideal for next generation sequencing (NGS)
- Cost-effective: No longer requires an additional NGS library prep step
- Compatible with Illumina® next-generation sequencing
Components
Other Notes
- A 20 μL Amplification 2 reaction will produce >100 ng of amplified double-stranded cDNA when starting with 100 pg to 5 ng of high-quality DNA. Higher input quantities and higher quality DNA template generally result in increased yields. For damaged DNA, such as from FFPE, 1–50 ng input DNA is recommended.
- The dual index adapter primers (AP100) provided in this kit will only work for one sample. If pooling of samples for sequencing is required, the user must provide additional index primer sets. See example index primer sequences on page 2 of the technical bulletin.
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Articles
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
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