Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

AC-6000

Sigma-Aldrich

HighPrep™ PCR Clean-up System

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
41105500

form

liquid

packaging

pkg of 250 mL (AC-60250 (HighPrep™ PCR (250 mL)))
pkg of 5 mL (AC-60005 (HighPrep™ PCR (5 mL)))
pkg of 50 mL (AC-60050 (HighPrep™ PCR (50 mL)))
pkg of 500 mL (AC-60500 (HighPrep™ PCR (500 mL)))

storage condition

do not freeze

storage temp.

2-8°C

General description

Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.

The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.

Application

HighPrep™ PCR Clean-up System may be used in:
  • Post-PCR and post-enzymatic reaction clean-up
  • NGS library preparation
  • Amplicon size selection for NGS
  • Microarrays
  • PCR
  • Sanger sequencing
  • Restriction enzyme digestions, adapter ligations
  • Cloning

Features and Benefits

  • High recovery of >100 bp amplicons
  • Adaptable to high throughput liquid handling systems
  • Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
  • No centrifugation or filtration leads to higher and purer yields

Storage and Stability

14 months when stored as directed

Legal Information

HiPrep is a trademark of Cytiva
HighPrep is a trademark of MagBio Genomics, Inc.

Disclaimer

For Research Use Only. Not for Use in Diagnostic Procedures.

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H361f

Hazard Classifications

Repr. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Shuba Varshini Alampalli et al.
PloS one, 12(12), e0188603-e0188603 (2017-12-09)
India has the third largest number of HIV-1-infected individuals accounting for approximately 2.1 million people, with a predominance of circulating subtype C strains and a low prevalence of subtype A and A1C and BC recombinant forms, identified over the past
The T-shirt microbiome is distinct between individuals and shaped by washing and fabric type
Sterndorff EB, et al.
Environmental Research, 109449-109449 (2020)
Eva Baggesgaard Sterndorff et al.
Environmental research, 185, 109449-109449 (2020-04-12)
Activity of the microbial population in clothing causes unpleasant odor and textile deterioration. However, little is known about how the textile microbial community is shaped. In this study, we developed a method for extracting DNA from small amounts of detergent-washed
Lauren Bowen Reoma et al.
Annals of neurology, 86(6), 878-884 (2019-10-11)
JC virus (JCV) infection is a lytic infection of oligodendrocytes in progressive multifocal leukoencephalopathy; less common forms of central nervous system manifestations associated with JCV infection include granule cell neuronopathy, encephalopathy, and meningitis. Presented is the first case of fatal
Soil bacterial diversity under conservation agriculture-based cereal systems in Indo-Gangetic Plains
Choudhary M, et al.
3 Biotech, 8, 304-304 (2018)
View All Related Papers

Protocols

HighPrep™ PCR Clean Up Kit and Purification System Protocol

HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service