DCH-012 human cell line

IHW Number: 9187, 94082287

• UNSPSC Code: 41106514

Properties

• biological source: human blood
• ethnicity: Thai
• gender: male
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice

Description

General description

Consanguineous: No

Homozygous: No

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 68 33
B: 08 46
HLA DRB1: 0301 09
HLA DRB3: 202
HLA DRB4: 101
HLA DQAP: 3.2 4.1
HLA DQA1: 0501 0302
HLA DQB1: 0201 0303
HLA DPB1: 0501 1701

Serological Profile

HLA A: 28 33
HLA A ief:
HLA C: 1 7
HLA B: 8 46

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.