C3710
Ciliary Neurotrophic Factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
CNTF
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product name
Ciliary Neurotrophic Factor human, CNTF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤325 ng/mL ED50 ((≥ 3.1 x 103 units/mg))
quality
endotoxin tested
mol wt
22.8 kDa
packaging
pkg of 10 and 20 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CNTF(1270) , CNTF (1270)
General description
The CNTF (ciliary neurotrophic factor) gene encodes a cytokine, and is mapped to human chromosome 11q12.1.
Application
Ciliary Neurotrophic Factor human has been used to study its effect on photoreceptor neuroprotection and Muller glial cell proliferation in zebrafish retina. It has also been used to study the effects of intravitreal injection of ciliary neurotrophic factor (CNTF) on photoreceptor injury and BRB (blood-retinal barrier) breakdown in transgenic model.
Biochem/physiol Actions
Ciliary neurotrophic factor (CNTF) was first identified as a survival factor for neurons from the ciliary ganglion of chicken embryos. Most of its known actions are restricted to cells of the nervous system, including motor neurons, sympathetic ganglion neurons, sensory neurons, hippocampal neurons, and medial septal neurons. CNTF also prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation. Outside the nervous system, CNTF maintains embryonic stem cells in an undifferentiated, pluripotent state. CNTF is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). CNTF exerts its actions through the activation of the high-affinity CTNF receptor complex, which contains the ligand-binding α-subunit (CNTF Rα) and two signal transducing β-subunits (LIF Rβ and gp130). The LIF Rβ subunit is also shared by receptors for LIF and OSM. The gp130 subunit is also shared by receptors for LIF, OSM, IL-6, and IL-11. CNTF is localized in the cell nucleus subsequent to receptor binding. Human and rat CNTF share ~83% sequence homology and show cross-reactivity in bioactivity.
Physical form
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Analysis Note
The proliferative activity is tested in a cell proliferation assay using the cytokine-dependent human erythroleukemic cell line, TF-1.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Genomic variants, genes, and pathways of Alzheimer's disease: an overview
Naj AC, et al.
American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics, 174(1), 5-26 (2017)
CNTF induces photoreceptor neuroprotection and Muller glial cell proliferation through two different signaling pathways in the adult zebrafish retina
Kassen SC, et al.
Experimental Eye Research, 88(6), 1051-1064 (2009)
Rachelle W Johnson et al.
Bone, 64, 47-56 (2014-04-12)
Muscle and bone are intimately linked by bi-directional signals regulating both muscle and bone cell gene expression and proliferation. It is generally accepted that muscle cells secrete factors (myokines) that influence adjacent bone cells, but these myokines are yet to
Weiyong Shen et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(45), 15715-15727 (2012-11-09)
Müller cells are the major glia of the retina that serve numerous functions essential to retinal homeostasis, yet the contribution of Müller glial dysfunction to retinal diseases remains largely unknown. We have developed a transgenic model using a portion of
Mansour Haidar et al.
Autophagy, 15(6), 1051-1068 (2019-01-24)
HSPB1 (heat shock protein family B [small] member 1) is a ubiquitously expressed molecular chaperone. Most mutations in HSPB1 cause axonal Charcot-Marie-Tooth neuropathy and/or distal hereditary motor neuropathy. In this study we show that mutations in HSPB1 lead to impairment
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