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A8312

Sigma-Aldrich

m-Aminophenylboronic acid–Agarose

aqueous suspension

Synonym(s):

(3-aminophenyl)boronic acid-Agarose, 3-Aminophenylboronic acid–Agarose, m-APBA-Agarose, m-Aminophenylboronic acid resin

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About This Item

MDL number:
MFCD00801303
UNSPSC Code:
12161501
PubChem Substance ID:
329770955
NACRES:
NA.32

form

aqueous suspension

Quality Level

300

extent of labeling

40-80 μmol per mL

matrix

6% beaded agarose

matrix activation

epichlorohydrin

matrix attachment

through amino to carboxyls of EDTA

matrix spacer

9 atoms

capacity

≥8 mg/mL binding capacity (peroxidase Type VI)

storage temp.

2-8°C

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General description

m-Aminophenylboronic acid (m-APBA) immobilized on an agarose gel is useful in immunoglobulins capture, but also leads to non-specific interactions. m-APBA is a boronate affinity matrix that is effectively used for affinity purification of monoclonal antibodies.

Application

m-Aminophenylboronic acid-Agarose has been used:
  • as a component of the column for the purification of Escherichia coli TOP10F cells
  • with cell lysates of various cells for detection of PARylated (Poly(ADP-ribos)ylation) proteins.
  • in the affinity purification of horseradish peroxidase (HRPO)
  • in the glycated human serum albumin (gHSA) purification

Physical form

Suspension in water containing 0.002% chlorhexidine diacetate.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Construction and optimization of a CC49-Based scFv-beta-lactamase fusion protein for ADEPT
Roberge M, et al.
Protein engineering, design & selection : PEDS, 19(4), 141-145 (2006)
RUNX poly (ADP-ribosyl) ation and BLM interaction facilitate the Fanconi anemia pathway of DNA repair
Tay L S, et al.
Testing, 24(7), 1747-1755 (2018)
T A Myöhänen et al.
The Biochemical journal, 197(3), 683-688 (1981-09-01)
The synthesis of 3-nitro-4-(6-aminohexylamido)phenylboronic acid is described. The properties of two novel forms of immobilized phenylboronate agarose adsorbents [m-aminophenylboronic acid-Matrex Gel and 3-nitro-4-(6-aminohexylamido)phenylboronic acid-Sepharose CL-6B] were investigated. Both gels bind and selectively retard the glycoprotein alpha-glucosidase from yeast. The retardation
Pravin K Bhatnagar et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 863(2), 235-241 (2008-02-09)
Hybrid hybridomas (quadromas) are derived by fusing at least two hybridomas, each producing a different antibody of predefined specificity. The resulting cell secretes not only the immunoglobulins of both parents but also hybrid molecules manifesting the binding characteristics of the
Monika Kijewska et al.
Molecules (Basel, Switzerland), 25(3) (2020-02-14)
We report herein a novel ChemMatrix® Rink resin functionalised with two phenylboronate (PhB) moieties linked on the N-α and N-ε amino functions of a lysine residue to specifically capture deoxyfructosylated peptides, compared to differently glycosylated peptides in complex mixtures. The
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