11218590910
Roche
DNA Molecular Weight Marker II, DIG-labeled
solution, pkg of 500 μL (10 μg/ml)
Synonym(s):
DNA marker
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About This Item
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form
solution
Quality Level
packaging
pkg of 500 μL (10 μg/ml)
manufacturer/tradename
Roche
greener alternative product characteristics
Designing Safer Chemicals
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sustainability
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concentration
10 μg/mL
color
colorless
solubility
water: miscible
greener alternative category
, Aligned
storage temp.
−20°C
General description
DNA Molecular Weight Marker II, DIG-labeled is a solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. This fragment mixture is prepared by cleavage of λDNA with Hind III. The size of the fragment ranges from 0.12 to 23.1 kbp. A photodigoxigenin has been introduced at approx. every 200th to 300th base in the DNA fragments.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
DNA Molecular Weight Marker II, DIG-labeled has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection.
Features and Benefits
Contents
Ready-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.
Ready-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.
Sequence
The mixture contains 8 fragments with the following base pair lengths: 125, 564, 2027, 2322, 4361, 6557, 9416, and 23130 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
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