11062590001
Roche
DNA Molecular Weight Marker VI
pkg of 50 μg (in 200 μl), solution
Synonym(s):
DNA marker
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About This Item
UNSPSC Code:
41105335
Recommended Products
form
solution
Quality Level
packaging
pkg of 50 μg (in 200 μl)
manufacturer/tradename
Roche
concentration
250 μg/mL
color
colorless
solubility
water: miscible
storage temp.
−20°C
General description
DNA Molecular Weight Marker VI is a fragment mixture prepared by cleavage of pBR328 DNA with restriction endonucleases Bgl I and pBR328 DNA digested with Hinf I. It has a size range of 0.15 to 2.1kbp.
The product is also available as DNA Molecular Weight Marker VI, Digoxigenin-labeled.
The product is also available as DNA Molecular Weight Marker VI, Digoxigenin-labeled.
Specificity
Specificity: Product has 5′-phosphorylated ends (restriction fragments).
Restriction site: Claevage sites and corresponding fragment lengths of pBR328:
- Bgl I cut sites at: 935, 1169, 2399, 4575, 4809 => creating following fragment sizes, respectively: 234 bp, 1230 bp, 2176 bp, 234 bp, 1033 bp.
- Hinf I cut sites at: 632, 852, 1006, 1304, 1757, 2274, 4040, 4434, 4732, 4886. =>
creating following fragment sizes, respectively: 220 bp, 154 bp, 298 bp, 453 bp, 517 bp,
1766 bp, 394 bp, 298 bp, 154 bp, 653 bp
Restriction site: Claevage sites and corresponding fragment lengths of pBR328:
- Bgl I cut sites at: 935, 1169, 2399, 4575, 4809 => creating following fragment sizes, respectively: 234 bp, 1230 bp, 2176 bp, 234 bp, 1033 bp.
- Hinf I cut sites at: 632, 852, 1006, 1304, 1757, 2274, 4040, 4434, 4732, 4886. =>
creating following fragment sizes, respectively: 220 bp, 154 bp, 298 bp, 453 bp, 517 bp,
1766 bp, 394 bp, 298 bp, 154 bp, 653 bp
Application
DNA Molecular Weight Marker VI is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
- Restriction digests
- PCR
- RT-PCR
Sequence
As determined by computer analysis of the pBR328 sequence, the mixture contains 15 DNA fragments with the following base pair lengths (1 base pair = 660 daltons) occuring once: 220, 394, 453, 517, 653, 1033, 1230, 1766, 2176 and fragments 154,234, and 298 occuring twice as a result of the digestion of pBR328 with both Bgl I and Hinf I.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Unit Definition
50 μg = 1A260 unit
Physical form
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
Once thawed we recommend further storage at 2 to 8 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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