528178

Plasminogen, EACA- and Lysine-Free, Human Plasma

• Synonym(s): Plasminogen, EACA- and Lysine-Free, Human Plasma, Profibrinolysin, Plasma Trypsinogen
• CAS Number: 9001-91-6
• UNSPSC Code: 12352202
• NACRES: NA.25
• MDL number: MFCD00131922
• eCl@ss: 42030240
• Specific activity: 7 units/mg protein

Properties

• description: Merck USA index - 14, 7521
• Quality Segment: 100
• form: lyophilized
• specific activity: 7 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native plasminogen (EACA- and lysine-free) from human plasma. Single chain glycoprotein containing 790 amino acid residues. Found in normal plasma at about 12 mg/100 ml. Inactive precursor of the serine protease plasmin. It is converted to the active protease by cleavage at Arg⁵⁶⁰.

Physical form

Lyophilized from 20 mM sodium phosphate buffer, 10 mg D-mannitol, 10 mg NaCl.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for at least 1 week at 4°C or for 1-2 months at -20°C.

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Other Notes

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of N-tosyl-Gly-Pro-Lys-pNA per min at 25°C, pH 7.8.

Sun, Z., et al. 2002. Biochim. Biophys. Acta1596, 182.
Robbins, K.C., et al. 1967. J. Biol. Chem.242, 2333.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3