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T3568

Sigma-Aldrich

TrypZean® bovine

recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP)

Sinónimos:

Trypsin bovine

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About This Item

Número de CAS:
9002-07-7
Comisión internacional de enzimas:
3.4.21.4 (BRENDA, IUBMB)
EC Number:
232-650-8
MDL number:
MFCD04041188
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in corn

Quality Level

300

form

lyophilized powder

specific activity

≥3,350 units/mg solid (USP)

UniProt accession no.

Q29463

storage temp.

−20°C

Gene Information

cow ... TRYP8(282603)

General description

TrypZean® eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.

Application

Eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsins.
TrypZean® has been used in a study to assess the use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells. TrypZean® has also been used in a study to compare a recombinant trypsin with the porcine pancreatic extract on sperm used for the in vitro production of bovine embryos.

Biochem/physiol Actions

For dissociation of cultured cells.

Preparation Note

Manufactured by Applied Biotechnology Institute utilizing proprietary transgenic plant protein expression system.

Other Notes

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

Legal Information

Trypzean is a registered trademark of ProdiGene, Inc.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000392048
0000392047
0000385424
0000385424
0000357909

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K J Mattson et al.
Theriogenology, 69(6), 724-727 (2008-02-19)
The objective was to compare the effects of treating bovine semen with two trypsin products (the porcine pancreas extract and a recombinant) and a control (no trypsin) on in vitro embryo production. Our hypothesis was that the trypsin treatments would
Pedro P Carvalho et al.
Cytotherapy, 13(5), 594-597 (2011-01-05)
Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells
240 recombinant bovine trypsin made in maize inactivates bovine herpes virus-1 adsorbed to the bovine Zona Pellucida
Seidel, G., et al.
Reproduction, Fertility, and Development, 19, 236-236 (2006)
Arun K Sharma et al.
Biotechnology advances, 27(6), 811-832 (2009-07-07)
In recent years, the use of plants as bioreactors has emerged as an exciting area of research and significant advances have created new opportunities. The driving forces behind the rapid growth of plant bioreactors include low production cost, product safety
Mian Zhou et al.
Nature, 495(7439), 111-115 (2013-02-19)
Codon-usage bias has been observed in almost all genomes and is thought to result from selection for efficient and accurate translation of highly expressed genes. Codon usage is also implicated in the control of transcription, splicing and RNA structure. Many
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