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N1531

Sigma-Aldrich

Denaturation Solution

for Northern and Southern blotting

Sinónimos:

Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer

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About This Item

Código UNSPSC:
12161700
NACRES:
NA.25

grado

for molecular biology

Nivel de calidad

200

Formulario

solution

temp. de almacenamiento

room temp

Descripción general

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Aplicación

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

Componentes

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

Otras notas

0.5 N NaOH, 1.5 M NaCl

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Pictogramas

Corrosion
GHS05

Palabra de señalización

Danger

Frases de peligro

H290,H314

Clasificaciones de peligro

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Código de clase de almacenamiento

8B - Non-combustible corrosive hazardous materials

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000340598
0000340598
0000301764
0000301763
0000257071

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José Manuel Pérez-Pérez et al.
Plant physiology, 134(1), 101-117 (2004-01-20)
The dwarf ucu (ultracurvata) mutants of Arabidopsis display vegetative leaves that are spirally rolled downwards and show reduced expansion along the longitudinal axis. We have previously determined that the UCU1 gene encodes a SHAGGY/GSK3-like kinase that participates in the signaling
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)

Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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