D9905
Denhardt′s Solution
for Northern and Southern blotting, lyophilized powder
Sinónimos:
Denhardt′s Solution For Blotting Technique
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About This Item
Código UNSPSC:
12161700
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
grado
for molecular biology
Nivel de calidad
Formulario
lyophilized powder
técnicas
Southern blotting: suitable
actividad extraña
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
temp. de almacenamiento
2-8°C
Descripción general
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Aplicación
Denhardt′s Solution has been used:
- as a component in hybridization buffer for the hybridization of brain sections[1]
- in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process[2]
- in the preparation of hybridization mixture[3]
Suitable as a blocking Buffer for Southern and Northern blot membranes.
Acciones bioquímicas o fisiológicas
Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.[4]
Componentes
Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).
Nota de preparación
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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