MAK128
Indican Assay Kit
sufficient for 100 colorimetric tests
Sinónimos:
Indoxyl Sulfate Assay
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
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usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
gastrointestinal diseases
storage temp.
−20°C
General description
Indican (indoxyl sulfate) is a putrefaction product resulting from bacterial deconjugation of tryptophan in the small intestine. Indican is water soluble and excreted through urine. Increases in urine indican can indicate protein maldigestion or malabsortion, or the overgrowth of gut anaerobic bacteria. Indican levels in urine can also increase in response to a high protein diet.
Application
Indican Assay Kit has been used to measure indican concentration in urine samples.
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
Suitability
The Indican Assay kit provides a simple and direct procedure for measuring indican levels in urine.
Principle
This assay is based on an improved Curzon and Walsh method in which the reaction of indican with p-dimethylaminobenzaldehyde results in a colorimetric product measured at 480 nm. This assay has a linear detection range between 0.2-20 mg/dL in the 96 well plate assay.
signalword
Warning
hcodes
Hazard Classifications
Met. Corr. 1 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
8B - Non-combustible corrosive hazardous materials
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Veit Rothhammer et al.
Nature medicine, 22(6), 586-597 (2016-05-10)
Astrocytes have important roles in the central nervous system (CNS) during health and disease. Through genome-wide analyses we detected a transcriptional response to type I interferons (IFN-Is) in astrocytes during experimental CNS autoimmunity and also in CNS lesions from patients
Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor.
Rothhammer V, et al.
Nature Medicine, 22(6), 586-586 (2016)
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