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L3126

Sigma-Aldrich

Lipase from porcine pancreas

Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

Sinónimos:

Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

Número de CAS:
9001-62-1
Comisión internacional de enzimas:
3.1.1.3 (BRENDA, IUBMB)
EC Number:
232-619-9
MDL number:
MFCD00131509
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Porcine pancreas

Quality Level

200

type

Type II

form

lyophilized (cake)

specific activity

≥125 units/mg protein (using olive oil (30 min incubation))
30-90 units/mg protein (using triacetin)

composition

protein, ≥20%

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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Application

Lipase from porcine pancreas has been used:
  • for the analysis of fatty acid at the 2-position of triglycerides.
  • to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.
  • as a component of dissolution media to study the dissolution profiles of theophylline.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Quality

Crude (Steapsin). Contains amylase and protease activity.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

Analysis Note

Protein determined by biuret.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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The contribution of fruits and vegetables to dietary intake of polyphenols and antioxidant capacity in a Mexican rural diet: Importance of fruit and vegetable variety.
Hervert-Hernandez D et al.
Food Research International, 44, 1182-1189 (2011)
M T Andonaegui et al.
Drug development and industrial pharmacy, 25(11), 1199-1203 (1999-12-22)
Dissolution profiles of theophylline (TP) from three types of sustained-release (SR) matrix tablets (plastic [PL], lipid [LP], and hydrophilic [HP]) in different dissolution media, with and without enzymes, were established. Also investigated was the influence of a treatment of the
Enzymatic transesterification of rapeseed oil and lauric acid in a continuous reactor.
Forssell, P., et al.
Journal of the American Oil Chemists' Society, 70, 1105-1109 (1993)
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Journal of pharmaceutical and biomedical analysis, 174, 486-494 (2019-06-24)
Natural compounds with diverse structural skeletons have different ionization efficiencies and different mass spectrometry (MS) responses. Some key factors influencing the electrostatic field induced spray ionization (EFISI)-MS for a variety of natural compounds have been optimized and improved. Fifteen reference
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