H3537

HEPES solution

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

• Sinónimos: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Número de CAS: 7365-45-9
• Número MDL: MFCD00006158
• Código UNSPSC: 12161700
• ID de la sustancia en PubChem: 329815199
• NACRES: NA.25

Propiedades

• Nombre del producto: HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered
• grado: BioPerformance Certified
• Nivel de calidad: 200
• esterilidad: 0.2 μm filtered
• Formulario: liquid
• concentración: 1 M
• técnicas: cell culture | mammalian: suitable
• impurezas: Bioburden, tested; DNase, RNase, Protease, Nickase, free; endotoxin, tested
• pH: 5.0-6.0
• intervalo de pH útil: 6.8-8.2
• trazas de catión: Fe: <5 ppm; heavy metals (as Pb): <5 ppm
• idoneidad: suitable for molecular biology
• aplicaciones: diagnostic assay manufacturing
• cadena SMILES: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Clave InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descripción

Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

RNAse HEPES has been used:

• To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
• To recover purified ribonucleotide
• As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
• As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable