81349

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt

free from low molecular weight nucleotides, lyophilized

• Sinónimos: Poly(dI-dC) sodium salt
• Número de CAS: 118578-37-3
• MDL number: MFCD00147947
• UNSPSC Code: 12352106
• NACRES: NA.52

Propiedades

• grade: for molecular biology
• Quality Level: 100
• form: powder
• quality: lyophilized
• mol wt: M_r ~2.5 × 10⁶ (broad molecular weight distribution); M_r ~2500000
• packaging: ampule of ≥10 units A₂₆₀
• storage temp.: −20°C

Descripción

General description

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.

Application

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.

Suitable for

• non-specific blocking agent in EMSA
• evaluation of DNA methyltransferases
• DNA-small molecule or drug interaction studies

Biochem/physiol Actions

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)