POLYDIC-RO
Roche
Poly[d(I-C)]
lyophilized, pkg of 10 U (10108812001 [A<sub>260</sub> units]), pkg of 50 U (11219847001 [A<sub>260</sub> units])
Sinónimos:
Poly-deoxy-inosinic-deoxy-cytidylic acid
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About This Item
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description
Poly-deoxy-inosinic-deoxy-cytidylic acid
Quality Level
form
lyophilized
mol wt
28814 bp
packaging
pkg of 10 U (10108812001 [A<sub>260</sub> units])
pkg of 50 U (11219847001 [A<sub>260</sub> units])
manufacturer/tradename
Roche
color
white
mp
54 °C (NaCl, 0.1 M, pH 7.0)
solubility
water: soluble
absorbance ratio
A290/260 nm 0.24-0.32
A280/260 nm 0.61-0.67
A250/260 nm 1.18-1.30
storage temp.
−20°C
Application
Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations.
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).
Features and Benefits
Contents
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence
Quality
Typical analysis:
Absorbance determination A250/A260, A280/A260, A290/A260
Absorbance determination A250/A260, A280/A260, A290/A260
Sequence
Chain Length approx. 20,000 bp
In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions.
As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.
As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.
Unit Definition
1 A260 unit corresponds to 50 μg ds DNA.
Unit Conversion: 1 A260 unit corresponds to 50 μg ds DNA.
Unit Conversion: 1 A260 unit corresponds to 50 μg ds DNA.
Preparation Note
Activator: Polymines (spermine, spermidine)
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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