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Merck
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Key Documents

76258

Sigma-Aldrich

Paraplast Plus®

suitable for electron microscopy, pellets (fast-melting)

Sinónimos:

Paraffin – polyisobutylene mixture

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About This Item

Número de CAS:
125387-89-5
EC Number:
232-315-6
MDL number:
MFCD00131795
UNSPSC Code:
12171500
NACRES:
NA.47

form

pellets (fast-melting)

Quality Level

100

technique(s)

electron microscopy: suitable

thickness

≤1 μm , short ribbons
≤2 μm , sectioning: continuous ribbons

mp

56-57 °C

application(s)

hematology
histology

storage temp.

2-8°C

General description

regular Paraplast with approx. 0.8% DMSO added for enhanced ease of infiltration and sectioning

Application

Paraffin polyisobutylene mixture is a tissue embedding medium composed of highly purified paraffin and polyisobutylene and a low level of dimethyl sulfoxide (DMSO) to enhance the rate of tissue penetration. Paraffin-polyisobutylene (76258) supports thin wrinkle-free sectioning down to 4 microM with more flexible and continuous ribbons.

Legal Information

Paraplast Plus is a registered trademark of Leica Biosystems

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Ewa Urbańczyk-Wochniak et al.
Cellular & molecular biology letters, 7(1), 7-18 (2002-04-11)
This study describes an effective method of in situ RT-PCR (RT-ISPCR) that was developed to localize gene expression in plant tissues. This RT-PCR technique was performed on sectioned tissues of female buds of the cucumber GY3 inbred line. The CUS1
H O Goyal et al.
The Anatomical record, 250(2), 164-171 (1998-03-07)
Since androgens and/or estrogens must bind with specific receptors in order to elicit a response at the target organ(s), it is important to understand factors that regulate expression of androgen receptors (AR) and estrogen receptors (ER). Hence, the objective of
R S Liem et al.
Acta anatomica, 132(2), 164-168 (1988-01-01)
A method is described for the histochemical detection of horseradish peroxidase in Paraplast Plus embedded brain sections. The procedure uses 150-micron-thick Vibratome-cut slices of glutaraldehyde-paraformaldehyde-fixed brain tissue. Tetramethylbenzidine stabilized by diaminobenzidine/cobalt/H2O2 is used as chromogen. The Vibratome-cut slices are dehydrated
Ye Jin et al.
The Plant cell, 21(7), 2072-2089 (2009-07-04)
Invertase plays multiple pivotal roles in plant development. Thus, its activity must be tightly regulated in vivo. Emerging evidence suggests that a group of small proteins that inhibit invertase activity in vitro appears to exist in a wide variety of
Jing Qi et al.
Plant physiology, 147(4), 1947-1959 (2008-06-20)
The size and shape of the plant leaf is an important agronomic trait. To understand the molecular mechanism governing plant leaf shape, we characterized a classic rice (Oryza sativa) dwarf mutant named narrow leaf1 (nal1), which exhibits a characteristic phenotype
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