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Millipore

Auto2D® 2-D Electrophoresis Device

automates 2D gel electrophoresis

Synonym(s):

2D Electrophoresis, 2DE, IEF, SDS-PAGE

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About This Item

UNSPSC Code:
41100000
NACRES:
NC.01

product line

Auto2D®

technique(s)

SDS-PAGE: suitable
electrophoresis: suitable
western blot: suitable

storage temp.

room temp

Related Categories

General description

The Auto2D® 2-D Electrophoresis Device automates two-dimensional gel electrophoresis. The applied sample enters the first-dimension gel passively, separating proteins by their isoelectric points. After equilibration, the first-dimension gel is set onto a horizontal SDS-PAGE gel, which resolves proteins by molecular weight.

Two-dimensional gel electrophoresis has generally been regarded as difficult to perform and time-consuming, requireing advanced user training while offering low reproducibility and high inter-operator variability.

Application

  • Differential proteomic analysis
  • Post-translational modification
  • Protein Isoforms
  • Western Blotting

Features and Benefits

Auto2D® 2-D Electrophoresis Device is user-friendly and enable two-dimensional electrophoresis in 1-2 hours for faster, more reproducible results.

  • Fully automated to eliminate tedious handling steps
  • Fast results in 1-2 hours
  • High reproducibility
  • Easy to use, with no advanced training required
  • Lower inter-operator variability

Storage and Stability

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Auto2D is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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Articles

Auto2D® Gel Electrophoresis Device

Auto2D® automates difficult 2D electrophoresis methods for protein localization with high reproducibility in under two hours.

Protocols

Auto2D® Automated 2-D Gel Electrophoresis Device Protocol

The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.

Introduction to SDS-PAGE - Separation of Proteins Based on Size

Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.

Related Content

Auto2D® 2-D Gel Electrophoresis Device Frequently Asked Questions

Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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