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796-0477

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)
mobile phase: [A] 20 mM Bis-Tris HCl, pH 7.0; [B] 20 mM Bis-Tris HCl, 0.5M NaCl, pH 7.0; 24 to 69% B in 20 min. (linear)
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 10 μg each variant

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

This Figure shows the separation of three cytochrome c variants on Discovery BIO PolyMA-SCX. Note the difference in elution order compared to the reversed-phase separation on Discovery BIO Wide Pore C18 shown in product G001581.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Standard

Referencia del producto
Descripción
Precios

C4011

Cytochrome c from pigeon breast muscle, ≥95% based on Mol. Wt. 12,173 basis

C2037

Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis

C3131

Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

C7150

Citocromo c from equine heart, BioReagent, suitable for GFC marker

C7752

Citocromo c from equine heart, ≥95% based on Mol. Wt. 12,384 basis

C2506

Citocromo c from equine heart, ≥95% (SDS-PAGE)

C2436

Cytochrome c from Saccharomyces cerevisiae, ≥85% based on Mol. Wt. 12,588 basis

C3483

Cytochrome c from human heart, ≥95% (SDS-PAGE), lyophilized powder

30398

Cytochrome c from bovine heart, ≥95% (GE)

C2867

Citocromo c from equine heart, BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

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