Product No. C2506 is prepared with trichloroacetic acid (TCA), whereas C7752 is prepared with the milder acetic acid. The TCA method may reduce the amount of superoxide dismutase (SOD) present, but tends to cause dimerization or acid-modified structures of cytochrome c. In contrast, acetic acid preparations may have slightly higher amounts of SOD, but a lower proportion of dimeric cytochrome c. So C2506 would be recommended for enzyme activity studies that require low SOD, whereas C7752 is recommended for structural studies or binding studies, or for enzyme activity studies where possible SOD contamination is not a problem. Product No. C2867 is prepared from C2506; it is a BioUtra grade product, greater than or equal to 99% pure as determined by SDS-PAGE. It is recommended when an ultra-high purity is required. Product No. C3256 is also prepared from C7752; it is isolated by ion-exchange chromatography. This fraction shows a high rate of ascorbic acid oxidation, but low activity in a cytochrome oxidase system. Product No. C7150 is also prepared from C7752; it is suitable for use as a gel filtration molecular weight marker.
C2867
Citocromo c from equine heart
BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture
Sinónimos:
Citocromo c from horse heart
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
10 mg | Comprobar disponibilidad del carrito | $243.00 |
50 mg | Envío estimado HOYdesdeMILWAUKEE | $886.00 |
Acerca de este artículo
biological source
horse heart
Quality Segment
product line
BioUltra
assay
≥99% (SDS-PAGE)
form
powder
mol wt
12,384
storage condition
(Tightly closed Dry)
technique(s)
cell culture | mammalian: suitable
solubility
water: 10 mg/mL, dark red-brown
UniProt accession no.
storage temp.
−20°C
Gene Information
horse ... CYCS(100053958)
General description
Application
Biochem/physiol Actions
Preparation Note
Other Notes
1 of 1
Este artículo | |||
|---|---|---|---|
| description BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture | description ≥95% based on Mol. Wt. 12,384 basis | description ≥95% (SDS-PAGE) | description ≥95% (SDS-PAGE), lyophilized powder |
| biological source horse heart | biological source horse heart | biological source horse heart | biological source human heart |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell based assay: suitable | technique(s) western blot (chemiluminescent): suitable, cell culture | mammalian: suitable, mass spectrometry (MS): suitable |
| assay ≥99% (SDS-PAGE) | assay ≥95% based on Mol. Wt. 12,384 basis, ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) |
| form powder | form powder | form powder | form lyophilized powder |
| mol wt 12,384 | mol wt 12,384 | mol wt 12,384 | mol wt 11 kDa by SDS-PAGE |
| storage condition (Tightly closed Dry) | storage condition - | storage condition - | storage condition - |
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Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Eyeshields, Gloves, type N95 (US)
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I see that you list several horse heart cytochrome c products. What are the differences, and which one should I choose?
1 answer-
Helpful?
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How should I store solutions of Product C2867, Cytochrome c?
1 answer-
Our general recommendations are as follows: Store at -20 °C (freezer) for up to six months. Store at 2-8 °C (refrigerator) for up to two weeks. Store at 20-25 °C (room temperature) for up to three days. According to J. Biol. Chem, 237, 2161-2174 (1962), cytochrome c is stable to boiling, and over an unusually wide range of pH values. However, it denatures readily at extreme pH (low or high), and in high concentrations of organic solvents, such as ethanol or acetone.
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How do I determine how much of Product C2867, Cytochrome c, is in the oxidized form, and how much is reduced?
1 answer-
This is determined by a spectral assay. The detailed assay procedure (operating procedure #11252) may be requested from Sigma St. Louis Technical Service (techserv@sial.com).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Is Product C2867, Cytochrome c, predominantly in the oxidized form or the reduced form?
1 answer-
This product is predominantly (>95%) in the oxidized form.
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