SRP5321

EIF2AK1 (HRI), active, GST tagged human

PRECISIO^®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Sinónimos: HCR, HRI, KIAA1369
• Número de CAS: 1418571-40-0
• UNSPSC Code: 12352200
• NACRES: NA.32

Propiedades

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• product line: PRECISIO^®
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 9.6-14.4 nmol/min·mg
• mol wt: ~120 kDa
• NCBI accession no.: NM_014413
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... EIF2AK1(27102)

Descripción

General description

Eukaryotic translation initiation factor 2-α kinase 1 (EIF2AK1) or HRI is a kinase that can be inactivated by hemin. The gene encoding this protein is localized on human chromosome 7p22.1.[1]

Biochem/physiol Actions

Eukaryotic translation initiation factor 2-α kinase 1 (EIF2AK1) modulates protein synthesis.[2] It acts at the level of translation initiation to downregulate protein synthesis in response to stress.[3] The protein is activated by heme deficiency and other stimuli and is a major protein kinase that phosphorylates EIF2-α.[2] EIF2AK1 is downregulated in the majority of ovarian cancers compared with normal ovarian tissues. It functions in iron homeostasis and may play a role in hemolytic and inflammatory anemia.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable