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SRP5232

Sigma-Aldrich

EIF2S1, His tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinónimos:

EIF-2, EIF-2α, EIF-2A, EIF2, EIF2A

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About This Item

Número de CAS:
480740-00-9
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

~40 kDa

NCBI accession no.

NM_004094

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... EIF2S1(1965)

General description

Eukaryotic translation initiation factor 2 subunit 1 α (EIF2S1) is a 35kDa protein. It is the α subunit of the eukaryotic initiation factor 2 (eIF2α). The gene encoding it is localized on human chromosome 14q23.3.

Biochem/physiol Actions

Eukaryotic translation initiation factor 2 subunit 1 α (EIF2S1) catalyzes the first regulated step of protein synthesis initiation and promotes the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. The Ser51 in the mature human EIF2-α protein acts as the sole site of phosphorylation that leads to repression of protein synthesis. EIF2-α also plays an essential role in erythropoiesis.

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2 alpha (eIF2a) dephosphorylation in mammalian development
Heather P
Proceedings of the National Academy of Sciences of the USA (2008)
Direct interaction between STAT3 and EIF2AK2 controls fatty acid-induced autophagy
Mireia N
Autophagy (2012)
Mechanism of activation of the double-stranded-RNA-dependent protein kinase, PKR
Krishna m
FEBS Journal (2001)
ER stress and autophagy contribute to CsA-induced death of malignant glioma cells
Iwona Anna
Autophagy (2012)
V K Pathak et al.
Molecular and cellular biology, 8(2), 993-995 (1988-02-01)
The phosphorylation of the alpha-subunit of initiation factor eIF-2 leads to an inhibition of protein synthesis in mammalian cells. We have performed site-directed mutagenesis on a cDNA encoding the alpha-subunit of human eIF-2 and have replaced the candidate sites of
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