This kit has been optimized with cell lysate and tissue lysate as the input sample types. If you are interested in a kit that can be used for cell culture supernatants, we recommend RAB0507 which is designed for serum, plasma, cell culture supernatants and urine samples.
RAB0508
Human VEGF ELISA Kit
for cell and tissue lysates
Sinónimos:
VEGF-A, Vascular endothelial growth factor A
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1 KIT
$569.00
$569.00
Normalmente se envía en 1 semana. (Para pedidos fuera de Estados Unidos y Europa, calcule la entrega 1 o 2 semanas más tarde)
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1 KIT
$569.00
About This Item
Código UNSPSC:
41116158
NACRES:
NA.32
$569.00
Normalmente se envía en 1 semana. (Para pedidos fuera de Estados Unidos y Europa, calcule la entrega 1 o 2 semanas más tarde)
Solicitar un pedido a granel
Productos recomendados
reactividad de especies
human
envase
kit of 96 wells (12 strips x 8 wells)
técnicas
ELISA: suitable
capture ELISA: suitable
entrada
sample type cell lysate
sample type tissue lysate
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL
método de detección
colorimetric
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Información sobre el gen
human ... VEGFA(7422)
Categorías relacionadas
Descripción general
The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in cell lysate and tissue lysate.
Inmunógeno
Recombinant Human VEGF-A
Aplicación
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Acciones bioquímicas o fisiológicas
VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis.[1] Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes.[2][3] Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease.[3] Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves′ disease, psoriasis, cardiovascular disease, and peripheral limb disease.[2]
Otras notas
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Met. Corr. 1
Código de clase de almacenamiento
8A - Combustible corrosive hazardous materials
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Los clientes también vieron
Suppression of retinal neovascularization in vivo by inhibition of vascular endothelial growth factor (VEGF) using soluble VEGF-receptor chimeric proteins.
Aiello LP
Proceedings of the National Academy of Sciences of the USA, 92, 10457-10461 (1995)
Vascular permeability factor/vascular endothelial growth factor: a multifunctional angiogenic cytokine.
Brown LF
EXS, 79, 233-269 (1997)
Miyako Kurihara-Shimomura et al.
International journal of oncology, 56(5), 1093-1100 (2020-04-23)
Mast cells (MCs) are present in the tumor stroma, and MCs that express the mast cell‑specific proteases tryptase and chymase (MCTC) exhibit several tumor‑related functions. It was previously reported that melanoma inhibitory activity (MIA) gene family members, including MIA, MIA2
Piyush Gondaliya et al.
PloS one, 13(11), e0208044-e0208044 (2018-11-30)
The role of DNA methylation has not been enough explored in pathophysiology of diabetic nephropathy (DN). However, according to recent reports it has been inferred that hypermethylation could be one of the principle cause associated with the enhancement of DN.
A common polymorphism in the 5'-untranslated region of the VEGF gene is associated with diabetic retinopathy in type 2 diabetes.
Awata T
Diabetes, 51, 1635-1639 (2002)
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can this kit be used for cell culture supernatants as well? if the same sensitivity is required and if the lysis buffer is not used...
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