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N2415

Sigma-Aldrich

Nucleoside Phosphorylase bacterial

recombinant, expressed in E. coli, ≥10 units/mg protein

Sinónimos:

PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase

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100 UNITS
$137.06
250 UNITS
$275.01
1000 UNITS
$886.44

$137.06

Precio de catálogo$154.00Ahorre 11 %

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100 UNITS
$137.06
250 UNITS
$275.01
1000 UNITS
$886.44

About This Item

Número de CAS:
9030-21-1
Comisión internacional de enzimas:
2.4.2.1 (BRENDA, IUBMB)
EC Number:
232-857-3
MDL number:
MFCD00131739
UNSPSC Code:
12352204
NACRES:
NA.54

$137.06

Precio de catálogo$154.00Ahorre 11 %

En stockDetalles

recombinant

expressed in E. coli

Quality Level

200

assay

≥80% protein basis (biuret)

form

lyophilized powder

specific activity

≥10 units/mg protein

sequence note

MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE

UniProt accession no.

P0ABP8

storage temp.

−20°C

SMILES string

[n]2(c3ncncc3nc2)[C@@H]1O[C@@H]([C@H]([C@H]1O)O)CO

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

InChI key

MRWXACSTFXYYMV-FDDDBJFASA-N

Gene Information

Escherichia coli ... deoD(945654)

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Application

Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.
Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.[1][2]
Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.

Biochem/physiol Actions

Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway.[3] It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.[4]

Unit Definition

One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.

Preparation Note

This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000378370
0000371480
0000378370
0000371480
0000298782

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Conformational stability and catalytic activity of PTEN variants linked to cancers and autism spectrum disorders
Johnston S B and Raines R T
Biochemistry, 54(7), 1576-1582 (2015)
Patricia Calero et al.
Nature communications, 11(1), 5045-5045 (2020-10-09)
Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture and materials. Addition of fluorine to organic structures represents a unique strategy for tuning molecular properties, yet this atom is rarely found in Nature and
Primary T-cell immunodeficiencies
Clinical Immunology (Orlando, Fla.), 54(7), 489-508 (2019)
Irina V Varizhuk et al.
Current protocols, 2(1), e347-e347 (2022-01-21)
A simple and efficient method for the preparation of α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate, key intermediates in nucleoside metabolism and important starting compounds for the enzymatic synthesis of various modified nucleosides, has been proposed. It consists in near-irreversible enzymatic phosphorolysis
Sean B Johnston et al.
PloS one, 10(1), e0116898-e0116898 (2015-01-22)
Phosphatase and tensin homologue deleted from chromosome ten (PTEN) is a lipid phosphatase tumor suppressor that is lost or inactivated in most human tumors. The enzyme catalyzes the hydrolysis of phosphatidylinositol-(3,4,5)-trisphosphate (PIP3) to form phosphatidylinositol-(4,5)-bisphosphate (PIP2) and inorganic phosphate. Here
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