A5168

Adenosine Deaminase from bovine spleen

Type IX, ammonium sulfate suspension, 150-200 units/mg protein

• Sinónimos: Adenosine aminohydrolase
• Número de CAS: 9026-93-1
• Comisión internacional de enzimas: 3.5.4.4 (BRENDA, IUBMB)
• Número CE: 232-817-5
• Número MDL: MFCD00081287
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bovine spleen
• Nivel de calidad: 200
• tpo: Type IX
• Formulario: ammonium sulfate suspension
• actividad específica: 150-200 units/mg protein
• mol peso: 32.5-33 kDa
• Nº de acceso UniProt: P56658
• actividad extraña: 5′-AMP deaminase ≤0.01%; alkaline phosphatase ≤0.01%; guanase ≤0.01%; nucleoside phosphorylase ≤0.1%
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: cow ... ADA(280712)

Descripción

Descripción general

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.[1]

Molecular weight: 32.5-33 kDa

pI: 4.85

Aplicación

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine).[2] It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.[3]

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays [4]. Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Acciones bioquímicas o fisiológicas

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.[1]

Definición de unidad

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Forma física

Suspension in 3.2 M (NH₄)₂SO₄, 0.01 M potassium phosphate, pH 6.0

Nota de análisis

Protein determined by biuret.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)