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F4890

Sigma-Aldrich

Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse

clone RG-16, purified immunoglobulin, buffered aqueous solution

Sinónimos:

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RG-16, monoclonal

form

buffered aqueous solution

should not react with

pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Specificity

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Application

Monoclonal Anti-Rabbit Immunoglobulins-FITC antibody produced in mouse has been used in:
  • immunocytochemistry
  • immunohistochemistry
  • immunofluorescence

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Physical form

Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Increased synthesis of heparin affin regulatory peptide in the perforant path lesioned mouse hippocampal formation
Poulsen FR, et al.
Experimental Brain Research. Experimentelle Hirnforschung. Experimentation Cerebrale, 135(3), 319-330 (2000)
Carole Ntayi et al.
The Journal of investigative dermatology, 122(2), 256-265 (2004-03-11)
Type I collagen mediates melanoma cells invasion through upregulation of matrix metalloproteinases-1 and -2 (MMP-1 and -2) expression and activation. We investigated here the contribution of elastin-derived peptides (ED), degradation products of elastin, the main component of elastic fibers in
Timofey Lebedev et al.
Oncogene, 40(44), 6258-6272 (2021-09-25)
Neuroblastoma (NB) has a low frequency of recurrent mutations compared to other cancers, which hinders the development of targeted therapies and novel risk stratification strategies. Multikinase inhibitors have shown potential in treating high-risk NB, but their efficacy is likely impaired
Kunio Hieshima et al.
Journal of immunology (Baltimore, Md. : 1950), 180(2), 931-939 (2008-01-08)
Adult T cell leukemia is a mature CD4+ T cell malignancy which predominantly expresses CCR4 and is etiologically associated with human T cell leukemia virus type 1 (HTLV-1). Because HTLV-1 transmission depends on close cell-cell contacts, HTLV-1-infected T cells may
A study of the process of apoptosis in animals infected with the contagious ecthyma virus
Garrido-Farina GI, et al.
Veterinary Microbiology, 129(1-2), 28-39 (2008)
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