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MilliporeSigma

B6916

Supelco

Reactivo Bradford

for 0.1-1.4 mg/ml protein

Sinónimos:

Ensayo de unión de las proteínas al colorante Coomassie, reactivo colorante proteico

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About This Item

UNSPSC Code:
12161500
NACRES:
NA.32

Quality Level

form

solution

storage temp.

2-8°C

General description

El análisis de Bradford consiste en añadir azul brillante de Coomassie G-250 a la disolución proteica. El colorante azul Coomassie se asocia con los aminoácidos básicos y aromáticos, causando de este modo un viraje en la absorbancia durante la determinación de proteínas.

Application

El reactivo de Bradford se ha utilizado para determinar la concentración total de proteínas.

Features and Benefits

  • El reactivo está listo para usar. No se requiere mezcla ni dilución.
  • La aparición del color es rápida. Bastan 5 minutos de incubación y la muestra puede leerse a 595 nm.
  • Los azúcares reductores y las sustancias reductoras junto con los tioles no interfieren con este reactivo.
  • El reactivo es adecuado para microanálisis (1 - 10 μg/ml) y análisis convencionales (50-1400 μg/ml).
  • Puede utilizarse en análisis de placas de micropocillos.
  • Análisis barato.

Legal Information

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pictograms

Health hazardCorrosion

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

target_organs

Eyes,Central nervous system

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sin-Jin Li et al.
Clinical nutrition (Edinburgh, Scotland), 36(3), 760-767 (2016-06-28)
The cellular mechanisms of obesity-induced cardiomyopathy are multiple and not completely elucidated. The objective of this study was to differentiate two obesity-associated cardiomyopathy miniature pig models: one with the metabolic syndrome (MetS), and one with a metabolically healthy obesity (MHO).
M Tal et al.
The Journal of biological chemistry, 260(18), 9976-9980 (1985-08-25)
Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their
Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
Charoenwong D et al.
Applied and Environmental Microbiology, 77, 5220-5229 (2011)
Influence of condensing equipment and temperature on exhaled breath condensate pH, total protein and leukotriene concentrations.
Czebe K et al.
Respiratory Medicine, 102, 720-720 (2008)
Hugh S Winwood-Smith et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(4), R347-R356 (2017-07-14)
Long-term studies have found that low-carbohydrate diets are more effective for weight loss than calorie-restricted diets in the short term but equally or only marginally more effective in the long term. Low-carbohydrate diets have been linked to reduced glycogen stores

Artículos

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

Protocolos

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Contenido relacionado

Métodos de cuantificación de proteínas, reactivos y tecnología de inmunoanálisis para la medición precisa de las concentraciones de proteínas en una variedad de muestras.

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

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