D5905

3,3′-Diaminobenzidine tetrahydrochloride

peroxidase substrate, chromogenic, tablet

• Sinónimos: 3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB
• Fórmula lineal: (NH₂)₂C₆H₃C₆H₃(NH₂)₂ · 4HCl
• Peso molecular: 360.11
• Beilstein/REAXYS Number: 1212988
• EC Number: 231-018-9
• MDL number: MFCD00012969
• UNSPSC Code: 12352204
• PubChem Substance ID: 24893981
• NACRES: NA.83

Propiedades

• Nombre del producto: 3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet
• Quality Level: 200
• form: tablet
• solubility: water: 1 tablet/15 mL (may contain small insoluble particles)
• storage temp.: −20°C
• SMILES string: Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
• InChI: 1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
• InChI key: KJDSORYAHBAGPP-UHFFFAOYSA-N

Descripción

Specificity

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Application

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.[1][2]

Reagent for spectrophotometric determination of selenium.

Biochem/physiol Actions

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.[3]

Reconstitution

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Other Notes

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Información de seguridad

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H302,H319,H341,H350
• pcodes: P202 - P264 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• ppe: Eyeshields, Gloves, type N95 (US)