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D5637
3,3′-Diaminobencidina tetrahydrochloride hydrate
≥96%
Sinónimos:
(1,1′-bifenil)-3,3′,4,4′-tetramina tetrahidrocloruro dihidrato
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 1 g | Fecha estimada de envío18 de mayo de 2026desdeMILWAUKEE | $51.50 | |
| 5 g | Fecha estimada de envío18 de mayo de 2026desdeMILWAUKEE | $172.00 | |
| 10 g | Fecha estimada de envío18 de mayo de 2026desdeMILWAUKEE | $303.00 | |
| 25 g | Fecha estimada de envío18 de mayo de 2026desdeMILWAUKEE | $680.00 | |
| 50 g | Fecha estimada de envío18 de mayo de 2026desdeMILWAUKEE | $1,130.00 |
Acerca de este artículo
Fórmula lineal:
C12H14N4 · 4HCl · xH2O
Número CAS:
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
231-018-9
MDL number:
Servicio técnico
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Permítanos ayudarleQuality Level
assay
≥96%
form
powder, crystals or chunks
technique(s)
titration: 96-104% (with NaOH)
color
white to light brown, and Faint Red and Faint Grey to Light Grey
mp
280 °C
solubility
H2O: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
InChI key
DXWSCXIZIHILNP-UHFFFAOYSA-N
General description
El tetrahidrocloruro de 3,3′-diaminobencidina hidratado es un cromógeno comúnmente utilizado en inmunohistoquímica y otras técnicas de tinción para visualizar la presencia de antígenos o anticuerpos en muestras biológicas. El HCl de DAB está diseñado como un colorante inmunohistológico para visualizar la actividad peroxidasa en las células. La oxidación de la DAB puede medirse utilizando un espectrofotómetro.
Application
El tetrahidrocloruro de 3,3′-diaminobencidina hidratado es adecuado para procedimientos de tinción inmunohistológica, inmunoelectrotransferencia y FISH (hibridación fluorescente in situ). La DAB se utiliza en el estudio de la apoptosis de células epiteliales en caso de cataratas tempranas, y en protocolos de inmunohistoquímica de la enfermedad renal poliquística autosómica dominante (PKD).
Biochem/physiol Actions
En la reacción de la peroxidasa, la DAB sirve como donante de hidrógeno en presencia de peróxido. La DAB oxidada forma un complejo insoluble de color marrón.
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Este artículo | |||
|---|---|---|---|
| technique(s) titration: 96-104% (with NaOH) | technique(s) - | technique(s) - | technique(s) UV/Vis spectroscopy: suitable |
| color white to light brown, and Faint Red and Faint Grey to Light Grey | color off-white to faint red, to beige | color - | color - |
| form powder, crystals or chunks | form powder | form powder | form - |
| solubility H2O: 50 mg/mL | solubility water: 50 mg/mL, clear to slightly hazy | solubility acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy | solubility - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
Clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Artículos
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| D5637-50G | 04061835347506 |
| D5637-100G | 04061832002095 |
| D5637-1G | 04061835347483 |
| D5637-10G | 04061835347476 |
| D5637-25G | 04061835347490 |
| D5637-5G | 04061835347513 |
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What is the Department of Transportation shipping information for this product?
1 answer-
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Do you have a staining protocol for Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 answer-
Dissolve 10 mg in 15 mL of Tris buffered saline, pH 7.6 (Tris-buffered saline tablet, Sigma Product No. T5030 may be used). Add 12 uL of fresh 30% hydrogen peroxide (Sigma Product No. H1009) prior to use. Solution may be filtered through a 0.2 micron filter immediately prior to use if necessary. DAB and hydrogen peroxide concentrations may be adjusted to suit individual applications. The stain can be enhanced with Ni. Make up 0.3% (w/v) NiCl2 in water. Add 1 mL of the NiCl2 stock to 9 mL DAB solution.
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What is the solution stability of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
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In our experience, solutions of DAB should be made immediately prior to use for best results. If and only if necessary, solutions can be kept in the dark and refrigerated for overnight storage. Based on our Immunology Department’s experience, storing the solution overnight did not give good reproducibility. However, the literature indicates that it is possible to store solutions of DAB at -20°C. DAB dissolved in Tris buffered saline (pH 7.6) at 0.6 mg/ml was reported to be stable for several weeks at 4°C.
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How is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), shipped and stored?
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The product is shipped at ambient temperature. However, it is stored at -20 degrees C for long term storage.
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Can the solutions of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), be filtered?
1 answer-
DAB solution may be filtered through a 0.2 micron filter immediately prior to use.
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What is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), soluble in?
1 answer-
DAB HCl is soluble in water at 50 mg/ml yielding a clear to slightly hazy solution. For consistency, it is important to allow DAB to come to room temperature before making solutions. Water quality is extremely important, so only high quality deionized or distilled water should be used to make DAB solutions.
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What is the shelf life of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?
1 answer-
This product has a shelf life of 3 years.
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